arXiv:2507.01339v1 Announce Type: cross Abstract: Music source separation (MSS) aims to extract individual instrument sources from their mixture. While most existing methods focus on the widely adopted four-stem separation setup (vocals, bass, drums, and other instruments), this approach lacks the flexibility needed for real-world applications. To address this, we propose GuideSep, a diffusion-based MSS model capable of instrument-agnostic separation beyond the four-stem setup. GuideSep is conditioned on multiple inputs: a waveform mimicry condition, which can be easily provided by humming or playing the target melody, and mel-spectrogram domain masks, which offer additional guidance for separation. Unlike prior approaches that relied on fixed class labels or sound queries, our conditioning scheme, coupled with the generative approach, provides greater flexibility and applicability. Additionally, we design a mask-prediction baseline using the same model architecture to systematically compare predictive and generative approaches. Our objective and subjective evaluations demonstrate that GuideSep achieves high-quality separation while enabling more versatile instrument extraction, highlighting the potential of user participation in the diffusion-based generative process for MSS. Our code and demo page are available at https://yutongwen.github.io/GuideSep/